FastStart Universal SYBR Green Master (Rox), 2x concentrated master mix that contains FastStart Taq DNA Polymerase, Reaction Buffer, Nucleotides (dATP, dCTP, dGTP, dUTP), SYBR Green I, and a reference dye.
Function test: Each lot is tested for performance in qPCR using three templates: a GC-rich template, an AT-rich template, and a long template (approximately 440 bp).
FastStart Universal SYBR Green Master (Rox) can be used for the
amplification and detection of any DNA or cDNA target, including those
that are GC- or AT-rich.
Combine this master mix with Transcriptor
First Strand cDNA Synthesis Kit (Roche) to achieve excellent results in
two-step qRT-PCR.
• Improve PCR sensitivity and specificity.
Minimize the formation of nonspecific amplification products.
• Avoid over-estimation of qPCR results.
Eliminate nonspecific amplification products and primer-dimers that increase the amount of bound quantified SYBR Green I.
• Amplify and detect a broad range of DNA or cDNA targets.
Amplify fragments up to 500 bp long, including those that are GC- or AT-rich.
• Save time and effort in qPCR preparation.
Eliminate the need to mix components, titrate MgCl2, or perform other time-consuming optimization steps.
• Prevent false positives resulting from carryover contamination.
Use
this dUTP-containing mix with Uracil-DNA Glycosylase to eliminate
contaminating DNA carried over from previous PCR reactions.
FastStart Universal SYBR Green Master (ROX); Instructions For Use
SYBR Green I is a DNA double-strand-specific dye. During each
phase of DNA synthesis, the SYBR Green I dye, which is included in the
reaction mix, binds to the amplified PCR products; the amplicon can be
detected by its fluorescence.
Hot start protocols have been shown to
significantly improve the specificity, sensitivity, and yield of PCR.
Heat-labile blocking groups on some of the amino acid residues of
FastStart Taq DNA Polymerase make the modified enzyme inactive at room
temperature (+15 to +25°C). Therefore, there is no elongation during the
period when primers can nonspecifically bind. FastStart Taq DNA
Polymerase is activated by removing the blocking groups at a high
temperature (i.e., a pre-incubation step at +95°C).
Universal ready-to-use hot start reaction mix for qPCR and
RT-qPCR on all real-time PCR systems requiring normalization with ROX.
FastStart
Universal SYBR Green Master (Rox) includes a novel reference dye that
enables its use on all real-time PCR instruments requiring normalization
with ROX, without modifications or adjustments to the specific
instrument or protocol. This ready-to-use, 2x concentrated master mix
contains all reagents (except primers and template) needed for running
quantitative, real-time DNA detection assays, including qPCR and
two-step qRT-PCR, in the SYBR Green I detection format. FastStart
Universal SYBR Green Master (Rox) generates excellent results on
instruments such as the Applied Biosystems 7900 HT Fast Real-Time PCR
System or the Applied Biosystems 7500 Real-Time PCR System. This product
is not intended for use with LightCycler® Instruments
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注:本站商品信息均来自于厂商,其真实性、准确性和合法性由信息拥有者(厂商)负责。本站不提供任何保证,并不承担任何法律责任。
注:本站商品信息均来自于厂商,其真实性、准确性和合法性由信息拥有者(厂商)负责。本站不提供任何保证,并不承担任何法律责任。
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